How Does the Human Chorionic Gonadotropin (HCG) ELISA Kit Work?

Posted by anna on February 26, 2022

The human chorionic gonadotropin (HCG) ELISA kit works on the sandwich principle. After a sample is collected, a microtiter well is coated with a monoclonal antibody directed against a specific antigenic site on the __HCG molecule. The sample is then incubated with an enzyme conjugate, consisting of an anti-HCG antibody coupled with horseradish peroxidase. Then the incubated samples are washed off. The amount of bound peroxidase is proportional to the concentration of HCG in the sample. After washing off the unbound conjugate, the resultant color will be measured. The intensity of the color is proportional to the hCG/HCG in the sample.

The hCG ELISA kit is used for the quantitative determination of Human hCG concentration in serum and urine. It contains three components: a 96-well plate, an anti-hCG antibody HRPO conjugate, and a TMB Substrate. In addition to the ELISA kit, you will need a deionized water-based microplate washer and precision pipettes.

The hCG ELISA kit has a sensitivity range of 0.02 mg/mL. The sensitivity range of this assay is between 0.5mIU/l. The EIA kit is convenient and time-saving. It also has a high sensitivity level of 100-1000IU/l. This test requires no analogues, which means it is not influenced by cross-reactivity.

The CTK hCG ELISA is a solid-phase enzyme-linked immunosorbent assay. It is used for the quantitative detection of human chorionic gonadotropin in human serum. The test includes two individual antibody conjugates, anti-human hCG antibody and anti-hCG HRPO conjugate. It requires a microplate washer and a deionized water source.

The hCG ELISA assay is a convenient and time-saving tool. It measures the hCG level in plasma, serum, and cell culture supernatants. It has the highest sensitivity of any available ELISA kit. It has been widely used in clinical trials and is useful for research. This ELISA assay is often used to detect human chorionic gonadotropin.


The hCG ELISA kit uses the DRG method to detect trisomy 21 in the first trimester. This test is a sensitive method that can help detect 90% of pregnancies with Down syndrome. When the DRG ELISA is used correctly, it can accurately determine the risk of a particular trisomy. In addition to detecting hCG levels, the DRG ELISA provides more detailed information about the hormone's role in pregnancy.

The hCG ELISA is a sensitive method for measuring hCG in the blood and urine. Its sensitivity is approximately 200 mIU/milliliter, which is six times higher than other hCG assays. As such, it is a useful tool for early pregnancy diagnosis. But it does not offer accurate results. If the result of the ELISA is not correct, it will be interpreted by a physician as a false negative.

Ferritin ELISA Kits

Human ferritin ELISA is a rapid and sensitive method for the measurement of ferritin levels in plasma. It is designed for the quantitative determination of ferritin levels in serum, milk, and plasma. This method is based on antibodies that have been designed to detect the minimal concentration of human-derived iron in the blood. This method is suitable for routine and research purposes. The sensitivity of this test is 0.5 ng/mL, and is comparable to commercial immunoassay systems.

The Ferritin ELISA is a sandwich ELISA. This kit measures the ferritin levels in plasma and serum. The reagents are compatible with recombinant and natural human-derived ferritin. A diluent is included in the kit. Although the suggested diluent is suitable for cell culture supernates, complex matrices such as agar must be evaluated prior to use.

The DuoSet ELISA Development kit contains the basic components for the development of sandwich ELISAs. The kit is suitable for measuring both natural and recombinant human-derived ferritin. In addition, the suggested diluent is suitable for cell culture supernat samples. However, the diluent must be evaluated prior to use. As a result, the DuoSet ELISA Development kit can measure both natural and recombinant human ferritin.

The DuoSet ELISA Development kit includes the basic components needed to develop a sandwich ELISA. This kit detects both recombinant and natural human ferritin. The suggested diluent is appropriate for most cell culture supernate samples. Moreover, the diluent should be evaluated before use in order to ensure that it is compatible with the sample matrix. This is necessary to ensure the accuracy of the results.

The Ferritin ELISA Assay Kit uses two monoclonal antibodies against ferritin. The ferritin from the samples and the standards bind simultaneously to the monoclonal antibody. Then, a washing step is performed to remove the unbound HRP conjugate. The enzyme substrate is added to the plate. The ELISA is finished once the absorbance of the two components reaches a certain threshold.

The Ferritin ELISA Assay Kit is designed to measure ferritin in human serum. It is intended for research use only. It is not intended for diagnostic use. In the case of human samples, it is essential to note that the ferritin ELISA assay kit should not be used for therapeutic purposes. This method has many limitations and is not recommended for every type of study. There are several advantages of this assay.

The enzyme immunoassay (ELISA) method is a convenient and accurate way to determine the concentration of ferritin in serum. It is a reliable tool for diagnosis of iron deficiency and to monitor the effects of iron. It is a cost-effective alternative for blood tests. In addition to being cost-effective, it has several advantages. If the sample is not of sufficient quality, the serum may not be toxic to the test subject.

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